Tuesday, March 31, 2015

Historical Contribution: 1971, Chung and Coffey, The Prostate Nuclei


1971
Biochemical characterization of prostatic nuclei. I. Androgen-induced changes in nuclear proteins L. W. Chung and D. S. Coffey Biochim Biophys Acta 1971 247: 570-83

 

Based on prior work, Dr. Coffey and colleagues demonstrated that the DNA, RNA and protein synthesis of the prostate gland varied with androgen levels (see the Historical Contribution: 1968). In this 1971 investigation (written in two parts), Drs. Chung and Coffey analyzed the nuclei and DNA content of rats following castration. They found that nuclei from prostate cells could easily be recovered in rats who were castrate (approximately 60%). However, in normal rats and those who received testosterone replacement (after castration) – the proportion of nuclei recovered was dramatically lower (13-15%). Thorough investigation determined a number of factors that contributed to the difference in prostate nuclei:
  1. Magnesium levels and the nuclear membranes are affected by testosterone levels. Aberrations in these normal cellular processes resulted in lower nuclei yield.
  2. Testosterone levels also affect the size of the nuclei (it had previously been demonstrated that testosterone affected the size of the whole prostate cell). Just as the prostate cell shrinks under castrate levels, so too does the prostate cell nucleus.
  3. Nuclear protein to DNA content (as measured by nuclear proteins and nuclear membrane proteins) is decreased with low testosterone levels and,
  4. … can be restored to normal after testosterone supplementation.


This series of elegant experiments can be found in Biochimica et Biophysica Acta. It is a wonderful example of Dr. Coffey's thoughtful and inventive approach to deciphering the prostate.

 

Visit the Centennial Website or click here to see more about the first 100 years at the Brady.

 


HISTORICAL CONTRIBUTIONS highlight the greatest academic manuscripts from the Brady Urological Institute over the past 100 years.  As the Brady Urological Institute approaches its centennial, we will present a HISTORICAL CONTRIBUTION from each of the past 100 years.  In the most recent experience, the most highly cited article from each year is selected; older manuscripts were selected based on their perceived impact on the field.  We hope you enjoy! 

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  20. كما يقوم فريق العمل بتنظيف الموكيت حيث أن الموكيت شركة تنظيف بدبي يعتبر من أهم الأشياء التي توجد في المنزل ويتميز بوجود الزخارف المختلفة شركة تنظيف بالعين به، لهذا فإنه يحتاج إلى التنظيف بكل دقة وحرص ويتم هذا من خلال استخدام شركة تنظيف عجمان الماء الفاتر والمنظفات المخصصة للموكيت، أو يتم تنظيفه عن طريق البخار شركة تنظيف كنب بدبي حتى يعطي أفضل النتائج وفي أسرع وقت
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  21. كما يقوم فريق العمل بتنظيف الموكيت حيث أن الموكيت شركة تنظيف بدبي يعتبر من أهم الأشياء التي توجد في المنزل ويتميز بوجود الزخارف المختلفة شركة تنظيف بالعين به، لهذا فإنه يحتاج إلى التنظيف بكل دقة وحرص ويتم هذا من خلال استخدام شركة تنظيف عجمان الماء الفاتر والمنظفات المخصصة للموكيت، أو يتم تنظيفه عن طريق البخار شركة تنظيف كنب بدبي حتى يعطي أفضل النتائج وفي أسرع وقت
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  22. تعمل على توفير كل خدمات عمليات التنظيف شركة تنظيف بالبخار بجدة بالإضافة إلى الخدمات الاخري شركة تنظيف كنب بالبخار بجدة التي تحتاجها ربات البيوت وأصحاب الشركات شركة تنظيف سجاد بالبخار بجدة والعقارات ومن خلال شركتنا المتمزية فى خدمات تنظيف المفروشات شركة تنظيف مجالس بالبخار بجدة بالبخار بافضل الاسعار تعرف على كافة شركة تنظيف بالبخار بمكة الخدمات المتميزة التى نقدمها شركة نقل عفش بمكة اليكن والى كل عملائنا الكرام

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  23. العمالة الفلبينية معروفة بأنها تتعلم شركة نقل عفش بجدة بسرعة كبيرة وجميع الأعمال التي تسند اليها تخرج جودتها بمستوى عالية فى حين ان مستوى الاجور لها منخفض وعليه فإن الشركة شركة تنظيف بالبخار بجدة تعمل على توفير تلك النوعية شركة تنظيف دكت المكيفات بمكة من العمالة ومن خلال افضل شركة نقل عفش بجده شركة تنظيف دكت المكيفات بجدة عمالة فلبينية سوف يتم الحصول على عمال متفوقين وقادرين شركة تنظيف دكت المكيفات بالطائف على نقل الاثاث بعناية كبيرة من اي مكان حتى الادوار العالية وتتم عملية النقل تبعا للأسلوب الحديث وهي كالتالي :-

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  26. ويتميز شركة تنظيف كنب بدبي بانجاز العمل بتقنية شركة تنظيف بدبي وجودة عالية، وفي أسرع وقت شركة تنظيف بالشارقة ،وتحرص شركة تنظيف بالعين بتوفير احدث شركة تنظيف بالعين الأجهزة الكهربائية شركة تنظيف بدبي والمعدات الحديثة اللازمة والمستخدمة في شركة تنظيف بعجمان عمليات التنظيف، وذلك حرصا من الشركة علي توفير شركة تنظيف كنب بدبي ومساعدة عملائها الكرام في القيام بإعمال

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  27. يمكنكم اللجوء لتنظيف منازل عجمان إذا شركة تنظيف دبي أصبحتم في حاجة إلى تنظيف المنزل شركة تنظيف عجمان في أي وقت تحددوه أنتم , فقط اتصلوا بتنظيف منازل عجمان شركة تنظيف بالشارقة وسوف يرد عليكم فريق متخصص من خدمة العملاء والذي يحدد معكم المطلوب شركة تنظيف ابو ظبي والوقت المحدد لإتمام تلك المهمة الضرورية وفي أسرع وقت ممكن سوف تجدونها انتهت شركة تنظيف كنب بدبي , فقط انسوا هم الأسعار التي تقلق الجميع في حالة الرغبة في التعامل مع أي شركة نظافة

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  32. I was diagnosed with stage 3 breast cancer in August of 2010. A precious friend told me about Dr Itua Herbal Center in West Africa,She gave me his contact number and email address,I contacted him quickly so he give me guaranty that his herbal medicine will cure my cancer and i will be heal forever I said Okay.I ask him about the process for the cure,he ask me to pay for the fees which i did and within 7 working days he sent me the herbal medicine then he instructed me on how to drink it for two weeks to cure.I told Gomez my friend about the herbal medicine so he gave me go ahead to drink it.So after drinking it for two weeks i was cured I'm so grateful and i promise i will recommend anyone with cancer to him and that what i'm doing.Dr Itua Herbal Medicine makes me believes there is a hope for people suffering from,Parkinson's disease,Schizophrenia,Cancer,Scoliosis,Bladder Cancer,Colorectal Cancer,Breast Cancer,Kidney Cancer,Leukemia,Lung Cancer,Skin Cancer,Uterine Cancer,Prostate Cancer,Fibromyalgia,a
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    Here Is His Contact Info......[Email...drituaherbalcenter@gmail.com/Whatsapp...+2348149277967]

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  33. If you have Herpes in your whole body such as fever blisters, hsv, or roofing shingles you know what it is like to stay with the pain and discomfort that herpes delivers. I don't need to tell you how awesome it would be to find something that works so well that you no longer need to fear about stress or outbreak,Dr Itua herbal medicine cure my herpes in two weeks of taking it.is genuine and natural herbal medicine it has no side effect, The reason I'm writing this is that I promised Dr Itua I will share his herbal work to the world to see Herpes is no more a big deal.also Dr Itua can as well cure the following diseases...HIV,Hsv 1/2,Hepatitis B,Cancer, Diabetes, Men/Woman Infertility, Scoliosis,Fibromyalgia,Fluoroquinolone Toxicity
    Syndrome Fibrodysplasia Ossificans Progressiva.Fatal Familial Insomnia Facator V Leiden Mutation , Lottery Spell,Copd, Shingles,Fibroid, Fibromyalgia, Liver/Kidney Inflamotry, Epilepsy, Coeliac disease,Creutzfeldt–Jakob disease,Cerebral Amyloid Angiopathy, Ataxia,Arthritis,Amyotrophic Lateral Sclerosis,Alzheimer's disease,Adrenocortical carcinoma.Asthma,Allergic Asthma. Here His Contact...drituaherbalcenter@gmail.com/Whatsapp Phone.+2348149277976. I paid for his herbal medicine and sent it to me through Courier Service which I pick it urgently and used.

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  34. دهان بالرياض
    معلم دهان بالرياض من الكوادر التي تعتمد عليهم الشركة في جميع أعمال الدهان والديكور، حيث يكون باستطاعته عمل أجمل الخلفيات والرسومات على الأسقف والحائط، وأيضاً يقوم بتركيب ورق الحائط بكافة أنواعه المختلفة والمتنوعة والذي يفضلها الكثيرون كبديل للدهانات.

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  35. HOW DR PERFECT ENLARGEMENT CREAM HELP ME TO ENLARGE MY PENIS YOU CAN ADD HIM UP ON WHATSAPP +1(256) 251 3614

    My name is David Richardson from USA I want to share a testimony of how Dr Perfect herbal mixture cream saved me from shame and disgrace, my penis was a big problem to me as the size was really so embarrassing and I was also having weak erection problem. I can't make love to my wife and my penis was just too small, a full grown man like me having 3 inches penis and to worsen it I don't last long in sex I can't even last four minutes it was really a big shame to me. My wife was really tired of me because my sex life was very poor, she never enjoyed sex, I was always thinking and searching for solutions everywhere until when I saw a testimony of how Dr Perfect herbal mixture cream have been helping people regarding their sex life, so I decided to give him a try and to my greatest surprise in less than one week of taking the herbs my penis grow to 10 inches I couldn't believe my eyes and as I speak now my penis is now 10 inches long and I don't have weak erection anymore. I can make love to my wife longer in bed. And my marriage is now stable my wife is now enjoy me very well in bed. All thanks to Dr Perfect for enlarging my penis he indeed save my marriage. You can contact him at solutiontoallproblems1@gmail.com or you can add him up on WhatsApp +1(256) 251 3614. you can also view my blog http://davidrichardson12356.over-blog.com. He also specializes in the following things
    Herpes virus
    Cancer
    Hsv
    Chronic venous disease
    and others

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  36. Unfortunately there are a lot of AIDS/Herpes denials on Herbal cures still out there. I did research on them after I was tested HIV/Herpes positive I was so worried am I going to die soon. I continue my search again on herbal remedy for Std, then I found lots of testimony on how Dr Itua Herbal Medicine Cured HIV/Aids, Herpes Virus,Copd, Hepatitis, Diabetes, On websites sharing their testimonies, which made much more sense to me. All the authors pronounce Dr Itua As a man with Good Heart, I pick interest in their testimonies and I contact him about my situation then he gave me procedure how it works, I proceed after one week he courier his Herbal Medicine to me and instruct me on how to drink it for two weeks to cure. I receive His Herbal Medicine so I drank it for two weeks as I was told then after 2 days I go for a test I found out I was cured from HIV/Aids & Herpes Virus, I pay homage to him 2 months ago to his country to celebrate with him on his African festival which he told me it usually happens every year. I know there are lots of (HIV)/Aids&Herpes Virus denials of Herbal Remedy movement the same few doctors and they represent a very small faction of the community. I could have died because I refused Natural Herbs Cures for so long, but luckily, by the grace of God I am alive to tell my story. Contact Info...Whatsapp Number...+2348149277967,Email...drituaherbalcenter@gmail.com/My Instagram Username...avat5634 Just in case you need someone to talk with. He cure tthe following diseases below...1. Herpes 2. cancer 3. HIV / AIDS 4. hepatitis 5. Bring my ex back 6. Leprosy 7. SARS 8. Bubonic plague
    9. Cerebrovascular disease 10. Lower respiratory infections 11. Syphilis 12. Influenza A-H1N1 (swine flu) 13.Ischemic heart disease 14. Chronic obstructive pulmonary disease 15. Whooping cough 16. Perinatal complications 17. diarrhea 18 .. lung cancer.

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  37. Irrespective of receiving daily oral or future injectable depot therapies, these require health care visits for medication and monitoring of safety and response. If patients are treated early enough, before a lot of immune system damage has occurred, life expectancy is close to normal, as long as they remain on successful treatment. However, when patients stop therapy, virus rebounds to high levels in most patients, sometimes associated with severe illness because i have gone through this and even an increased risk of death. The aim of “cure”is ongoing but i still do believe my government made millions of ARV drugs instead of finding a cure. for ongoing therapy and monitoring. ARV alone cannot cure HIV as among the cells that are infected are very long-living CD4 memory cells and possibly other cells that act as long-term reservoirs. HIV can hide in these cells without being detected by the body’s immune system. Therefore even when ART completely blocks subsequent rounds of infection of cells, reservoirs that have been infected before therapy initiation persist and from these reservoirs HIV rebounds if therapy is stopped. “Cure” could either mean an eradication cure, which means to completely rid the body of reservoir virus or a functional HIV cure, where HIV may remain in reservoir cells but rebound to high levels is prevented after therapy interruption.Dr Itua Herbal Medicine makes me believes there is a hope for people suffering from,Parkinson's disease,Schizophrenia,Cancer,Scoliosis,Fibromyalgia,Fluoroquinolone Toxicity
    Syndrome Fibrodysplasia Ossificans Progressiva.Fatal Familial Insomnia Factor V Leiden Mutation ,Epilepsy Dupuytren's disease,Desmoplastic small-round-cell tumor Diabetes ,Coeliac disease,Creutzfeldt–Jakob disease,Cerebral Amyloid Angiopathy, Ataxia,Arthritis,Amyotrophic Lateral Sclerosis,Alzheimer's disease,Adrenocortical carcinoma.Asthma,Allergic diseases.Hiv_ Aids,Herpe ,Copd,Diabetes,Hepatitis,I read about him online how he cure Tasha and Tara so i contacted him on drituaherbalcenter@gmail.com even talked on whatsapps +2348149277967 believe me it was easy i drank his herbal medicine for two weeks and i was cured just like that isn't Dr Itua a wonder man? Yes he is! I thank him so much so i will advise if you are suffering from one of those diseases Pls do contact him he's a nice man.

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  38. I am 29 years old and I was diagnosis of Breast cancer, easy well and have a similar story except my first acceptance as a Herbal medicine denials. I wasn’t into the movement Perseid and didn’t establish connections with any of them really, just had faith in believing how they work. I say this because it was during using Dr Itua herbal medicine i now then testify herbal medicine is real, Dr Itua herbal medicine cure my breast cancer I was suffering from for 2 years. Dr Itua herbal medicine is made of natural herbs which has no side effect and is easy to drink as well. Contact Dr Itua herbal Center if you are suffering from the same Breast Cancer or any kind of human Diseases including....HIV/AIDS, HERPES COLORECTAL CANCER, BLADDER CANCER, PROSTATE CANCER, KIDNEY CANCER, LUNG CANCER, SKIN CANCER, UTERINE CANCER, LEUKEMIA, VIRUS, HEPATITIS, WOMEN/MEN INFERTILITY, LOVE SPELL,LOTTERY SPELL. HIS CONTACT EMAIL/WHATSAPP: drituaherbalcenter@gmail.com/+2348149277967

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  39. I was diagnosed with stage 3 breast cancer in August of 2010. A precious friend told me about Dr Itua Herbal Center in West Africa,She gave me his contact number and email address,I contacted him quickly so he give me guaranty that his herbal medicine will cure my cancer and i will be heal forever I said Okay.I ask him about the process for the cure,he ask me to pay for the fees which i did and within 7 working days he sent me the herbal medicine then he instructed me on how to drink it for two weeks to cure.I told Gomez my friend about the herbal medicine so he gave me go ahead to drink it.So after drinking it for two weeks i was cured I'm so grateful and i promise i will recommend anyone with cancer to him and that what i'm doing.Dr Itua Herbal Medicine makes me believes there is a hope for people suffering from,Parkinson's disease,Schizophrenia,Cancer,Scoliosis,Bladder Cancer,Colorectal Cancer,Ovarian Cancer,Pancreatic Cancer,Breast Cancer,Kidney Cancer,Leukemia,Lung Cancer,Skin Cancer,Uterine Cancer,Prostate Cancer,Fibromyalgia,a
    Syndrome Fibrodysplasia ,Epilepsy Dupuytren's disease, Diabetes ,Coeliac disease, Angiopathy, Ataxia,Arthritis,Amyotrophic Lateral Sclerosis,Alzheimer's disease,Adrenocortical carcinoma.Asthma,Allergic diseases.Hiv_ Aids,Herpes,Inflammatory bowel disease ,Copd,Diabetes.
    Here Is His Contact Info......[Email...drituaherbalcenter@gmail.com/ info@drituaherbalcenter.com. Whatsapp...+2348149277967]

    ReplyDelete
  40. Useful article, thank you for sharing the article!!!

    Website: blogcothebanchuabiet.com chia sẻ những câu nói mỉa mai người khác hay stt một mình vẫn ổn và giải thích hiện tượng chim sẻ bay vào nhà là điềm gì.

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